THE VALIDATION OF THE METHOD FOR QUINOLONES RESIDUES DETERMINATION IN SAMPLES OF MILK, EGG POWDER, TISSUES, BLOOD PLASMA OF ANIMALS USING THE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY

The article presents the results of validation screening method for determination quinolone antibiotics residues (enrofloxacin, ciprofloxacin, marbofloxacin, danofloxacin) in tissue samples, egg powder, milk and blood plasma using RIDASCREEN®Chinolone/Quinolones test kits from R-Biopharm (Germany). According to information provided by manufacturer kit, quantitative quinolones content can be carried out following matrices: shrimp, fish, eggs, meat. In accordance with European Commission Decision 2002/657/EC, methods proposed were validated taking into account maximum permissible levels (MRLs), development sample preparation techniques assay liver, various representatives group corresponding presented.
 suitability technique was confirmed on basis establishing main parameters (technical threshold cut-off factor) control (blank) samples (muscle parenchymal tissues poultry animals, cow’s milk, broiler chickens powder) spiked standard solutions at level ½ MRL according "added-found" criterion. advantages developed are simplicity performance, rapidity economic efficiency.
 reliability obtained verified confirmatory UPLC-MS/MS method. comparative study chicken muscle tissues, powder enrofloxacin solution 2.5 5.0 μg/kg used withdrawal periods bioequivalence veterinary drugs based antibiotics.